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Assembly of the Marburg virus nucleocapsid involves interactions between the N- and C-terminal domains of the viral matrix protein VP40.
The Marburg virus (MARV) matrix (VP40) protein is a 280-residue type I membrane-anchored phosphoprotein essential for viral replication. We have previously shown that VP40 contains two independent structural domains, a C-terminal domain (CTD) that consists of a coiled-coil region and a serine/threonine-rich C-terminal tail (CTT) and a N-terminal domain (NTD), which contains a stretch of highly conserved basic residues. Here, we show that the central region of the NTD and the CTD are important for virus replication. In the absence of VP40, nucleocapsid (NC) formation is restricted to the N- and C-terminal domains. VP40 can be incorporated into the NC when fused to other proteins from other filoviruses; however, disruption of the N- and CTD or of the CTD alone severely affects

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