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Effect of potassium-channel blockers on the action potential in the rat neuroblastoma N1E-115 cell line.
Exposure of N1E-115 cells to the inhibitors of the different ion channels of the K+ conductance led to changes in the electrical activity of these cells. The action potentials recorded in a cell culture incubated with the K+-channel blocker TEA was a supramaximum depolarization which lasted for more than 10 min. In a Ca2+-containing solution the amplitude of the action potentials decreased gradually in the initial minutes of incubation with TEA. At the same time the rising phase of the action potential decreased in amplitude and the action potential became irregular. In the case of KCN, the inhibitor of the slow K+ channels, the action potential was shortened gradually by 15-20% during the first 20 min of incubation. The length of the action potential was not changed in N- and Ca2+-containing media. KCCO, a blocker of Ca2+-activated K+ channels, caused also a decrease in the rising phase of the action potential. However, the length of the action potential was shortened by 25-35% in both media. 4-AP, the blocker of the slow Cl- channels, reduced the amplitude of the action potential by about 20% in Na+-free and Ca2+-containing media. The length of the action potential was shortened by 8-10% in both media. It is concluded that in N1E-115 cells, the duration of the action potential is determined by the K+ conductance, the Cl- conductance and also the fast Na+ conductance.SOS response to high-ammonium stress in four discrete areas of the common bluefin tuna (Thunnus thynnus) liver.
This work was aimed to investigate a potential stress response of the common bluefin tuna (Thunnus thynnus) in the liver, which is the primary tissue for ammonium (NH(4)(+)) uptake and accumulation. The liver of adult fish (600-600 g) was investigated in four discrete areas: 1) the liver, 2) the gills, 3) the liver sinusoid and 4) the gall bladder. Samples were taken at 10 h in the period from 0 to 22 h (10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 110, 130, 150

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