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Cytoplasmic membranes are endowed with extraordinary biosynthetic, signaling and regulatory functions. Membranes receive and process external and internal signals, thereby acting as integral components of complex regulatory networks. Protists face huge fluctuations in their environment (variable light, temperature and salinity) and their cellular membrane systems are highly dynamic. The study of the immediate response to changes in physical conditions has so far been the domain of the eukaryotic model system Saccharomyces cerevisiae. In this review we will (i) outline a strategy for how to study the physiological response of a single cell to a change in physical conditions, (ii) describe how some of the pitfalls in the application of this strategy to higher organisms may be overcome and (iii) introduce a new model organism, the Trichomonas vaginalis parasite of the human urogenital tract, as a framework within which to study the immediate response of protists to changes in the environment. We will also discuss alternative techniques for the analysis of protist membrane systems and suggest
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