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Design EcoRaptor B v1.0.5 RC1 The Magazine.Application Information: Thymosin beta 4 (1-41) is intended for use as a diagnostic or research reagent.
Description:
Thymosin beta 4 is a naturally occurring peptide found in the thymus. It is a 43 amino acid polypeptide found in a variety of species, including humans, and is believed to play a role in the maturation of T-cells and to affect the function of immunocompetent cells. It has been proposed that thymosin beta 4 may play a role in cellular immune functions.
Thymosin beta 4 has been found to be associated with specific binding to a particular portion of the ganglioside GM3. GM3 has been identified to be a differentiation antigen associated with macrophages. Monoclonal antibody to GM3 can be used to distinguish the macrophage from the lymphocyte. This has proven useful in the identification of macrophages in the lymphoid tissues.
Thymosin beta 4 is an unglycosylated acidic peptide that has been purified from porcine thymus. It is produced by acidic hydrolysis, purification by gel filtration, followed by reverse phase high performance liquid chromatography. It has a molecular weight of 7,800 daltons as determined by sodium dodecyl sulfate electrophoresis.
The lyophilized powder, upon reconstitution, is provided as a sterile, pyrogen-free solution containing 1 mg/ml of thymosin beta 4. A stock solution of thymosin beta 4 has been demonstrated to be stable for at least one year at -20°C. Since thymosin beta 4 is a polypeptide and exhibits self-association characteristics, the lyophilized powder must be reconstituted using sterile, pyrogen-free water for injection (0.9% NaCl or 0.9% NaCl with 10% dextrose).
Thymosin beta 4 may be radiolabeled with either iodine-131 or tritium by the method of nucleophilic displacement. The radiolabeled thymosin beta 4 may be used to prepare immunoscintigraphic images of lymphoid tissues.
Instructions for use:
Reconstitute with 1 ml of sterile 0
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