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Jun 19, 2012 – Dangerous Ishq is scheduled for release on July 18. Karisma Kapoor, who plays the lead role, is a known name in.AgR and SplAT, regulators of atp-synthase subunit D: effects on transcript and protein levels in a thermophilic eubacterium.
Thermosomes, a subgroup of the ATP synthase, are constituted by a dimeric beta-sheet DNA binding protein, and two archaeal and eubacterial RNA-binding proteins, the ferredoxin-like transcription factor AgR, and the C-terminal domain of the splat (SplAT). The mechanism underlying the folding of these proteins, and more in general the stoichiometry of beta- and alpha-helical subunits of thermosomes, is unknown. Using a simple in vitro system, we have shown that the growth of the thermophilic archaeon Sulfolobus acidocaldarius is highly affected by the expression of the agr-dnaI operon, which encodes for the AgR and SplAT proteins. The introduction of dnaI-coding sequences in an agr- mutant strain restored the normal growth rate of the new recombinant, as did deletion of the agr- gene. When grown under conditions that are known to induce the production of atp-synthase, the agr- strain overproduced agr- and SplAT, but exhibited a lower abundance of the corresponding transcripts. Since these transcriptional effects of SplAT were
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