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In an attempt to understand the regulation of translation initiation, we studied the translocation of mTOR, p70 S6 kinase, 4E-BP1 and eIF4E in a cell-free system by using permeabilized and polysome-labeled rat skeletal muscle cells. We found that 4E-BP1 is mainly localized in the cytoplasm and is translocated into the nucleus by insulin. Furthermore, we show that insulin does not cause substantial phosphorylation of 4E-BP1 in the nucleus. However, insulin does cause phosphorylation of p70 S6 kinase and 4E-BP1 in the cytoplasm. Interestingly, insulin does not cause substantial phosphorylation of mTOR or eIF4E in the cytoplasm. These results suggest that 4E-BP1 may be a cytoplasmic target for insulin signaling.I’m in a tutoring session. Our last class was metaphysics, and I guess that’s a pretty good place to start this one.
In metaphysics, there are commonly two types of roads to the truth: reason and faith. The most important way to remember this, is that there are just two ways to get to the truth about God. There is simply no other.
When I was a kid, my mom used to say, “If you can’t find God, then you must create him.”
Once you realize that, it’s easy to see why theists get so angry. In a universe where there are simply two ways to get to the truth, the creationist has simply chosen not to go the metaphysically obvious, rational way
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