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1. Cells grown in high light have reduced sensitivity to ATP-inhibitors, including oligomycin, 2,4-dinitrophenol, and the protonophore, carbonyl cyanide m-chlorophenylhydrazone (CCCP). 2. The oligomycin sensitivity in high-light grown cells is consistent with the induction of oxidative phosphorylation (OXPHOS) in response to high-light. 3. Polypeptides with apparent molecular weights of approximately 50 000-94 000 which inhibit OXPHOS, as measured with isolated chloroplasts, were detected in high-light grown cells. Some of these polypeptides may be identical or similar to those previously found in low-light grown cells. 4. Antibodies to the 50-94 kDa polypeptides did not inhibit ATP synthesis or inhibit an ATP-mediated proton exchange reaction in the lipid-protein or disulfide-exchange assay with isolated chloroplasts, and did not inhibit ATP synthesis in assays using permeabilized cells, indicating that these polypeptides are not essential for ATP synthesis. 5. The persistence of the protein inhibitor from low-light to high-light growth and its identification by antibodies, suggests that ATP synthesis is repressed when cells are grown in high-light.Concentrations and Enteroparasite Risks in Commercial Swine Production Systems in the Southeastern United States.
The level of contamination of fecal material in swine feedlots has been associated with a variety of detrimental environmental effects on the health of the swine host and other species. The objectives of this study were to estimate the concentration of Enterobacteriaceae (E. coli, Salmonella) and other enteroparasites in the fecal and feeding materials of commercial swine operations in the southeastern US. A total of 5
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President Trump’s new education secretary, Betsy DeVos, has come under fire from the education community after a closed-door meeting with an anti-LGBT group.
DeVos’ invitation to the Council of Conservative Citizens (CCC), a group that has been identified by the Southern Poverty Law Center as an extremist hate group, was first reported by the Huffington Post, and was later confirmed by ABC News.
The New York Times reports that DeVos is scheduled to meet with CCC on Tuesday.
The Times notes that the CCC is an organization that “condemns interracial marriage and the legalization of abortion,” and that it doesn’t appear to have done away with overtly racist posts online, even after they have been criticized and removed. CCC, which has been around since 1964, also once claimed that “the NAACP is an evil, racist organization.” The group claims to have no official ties to DeVos but is apparently one of her top endorsements.
The Huffington Post reports that school district officials for the Michigan Education Association have been protesting the meeting between DeVos and the CCC, with one saying that it will prevent children in Michigan schools from learning about gay history. The MEA claims that it is promoting “fairness and equality” and is “the only public school teacher union in America.”
DeVos is no stranger to controversy surrounding her extreme positions on things like school choice and vouchers, but the Huffington Post notes that her inclusion of the anti-LGBT group will “erode the credibility of the entire education department.”
The CCC has supported people like anti-gay crusader Bryan Fischer, and has called for the impeachment of Supreme Court Justice Ruth Bader Ginsburg over her “homosexual conduct.”
The organization supports abstinence-only programs and opposes gay marriage, and has defended California’s Prop 8.Q:
socket.io is not defined
I have a problem using socket.io in my client side. I am trying to make a real time live chat. Everything is working except that the socket.io is not defined. I have included
In the client side (it is the last line in the body) and ran the following code.
var socket =