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Transocean – The Shipping Company
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. ships and employs more than 60,000 people throughout the world. As the world’s leading deep-water floating crane. per… INOG ..
TransOcean
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Transocean (Transatlantic Crossing)
Applying Transocean’s Guiding Principles to Global. Transocean has. Each order and exchange requires the use of its VARs to act as.. Data Transmission Service (DTS) solution.. Primary Data Transocean Inc. .
Transocean
TransOcean (TransAtlantic Crossing)
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TransOcean is a major ship management company that services vessels worldwide,. The Transocean Company is a wholly owned subsidiary of the Transocean .
Learn About TransOcean Online | TransOcean.com
transocean.com offers comprehensive information about TransOcean, and a searchable directory. This capability is available in ALL TransOcean packages, including TransOcean .[Cloning and expression of a low affinity IgE receptor gene in pig].
The aim of this study was to clone and express a cDNA encoding pig low affinity IgE receptor (pLAR), and find out whether the recombinant expressed pLAR could be recognized by anti-pig IgE. Firstly, a total RNA of pig peripheral blood cells was isolated, then a cDNA encoding the pLAR was amplified by a reverse transcription polymerase chain reaction (RT-PCR) with specific primers, and subcloned into a suitable expression vector. After identification by restriction endonuclease analysis and DNA sequence analysis, the plasmid was transformed into electrocompetent Escherichia coli cells and a colony was selected for further culture. The recombinant E. coli was cultured and the expressed protein was purified by affinity chromatography. Moreover, the anti-pig IgE was used to identify the recombinant pig pLAR. A cDNA encoding a full-length pLAR was obtained by RT-PCR with total pig-blood-cell RNA, and a recombinant pLAR protein was expressed in E. coli. The recombinant pLAR was recognized by anti-pig IgE. The cDNA encoding pLAR has been successfully cloned
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