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. bw of a soldier before he becomes a commandos behind enemylinesfdThe polymerase chain reaction (PCR) is the method of choice for a large number of laboratory and clinical diagnostic tests. Many of these tests call for multiple cycles of PCR amplification (denaturation, annealing, extension), as well as for the accumulation of the amplified material after each cycle to establish a detectable result. Because the PCR protocol is so robust, it can be carried out by any laboratory technician, even one with little experience. The repetitive nature of the PCR reaction exposes the operator to the risk of accidental infection by the amplified material. In the closed system of the laboratory, the risk may be mitigated by using automated pipetting robots or by employing laboratory procedures that minimize the volume of amplified material that contacts the operator. In the open system, the use of plastic or disposable containers and the provision of safe, readily decontaminated products are needed to minimize the exposure of the operator. The basic components of the PCR technique, including the instrumentation for PCR, are provided in most leading laboratories.
Automated systems for the PCR protocol employ a number of the reagents and reactions needed for the technique in disposable plastic containers. The containers are fitted with lids having one or more apertures to facilitate the introduction of specimens and the removal of products. Some of these containers are stable and readily reopenable. Others have a snap-fit lid that can be opened only by heat or ultrasonic energy. Both types require the operator to puncture a resilient membrane or otherwise disrupt the container to gain access to the specimen. Because of the risk of contamination, devices of this type are rarely used in open or clinical laboratories in the United States.
The PCR technique has typically been carried out in multiple-reaction tubes. These tubes may be open or closed, and may be made of glass, plastic or a composite of the two, but the tubes must have a very small volume for efficient handling. An experimental system employing in-tube PCR and a 48-reaction solution-array tube is described in U.S. Pat. No. 5,395,700. A similar device, employing 40 specimens in a 96-tube array, has been used successfully in the closed environment of a clinical diagnostic lab. Neither system has been adapted to the open environment, where the risk of contamination is so serious.
To satisfy the requirements of the open environment, a system has been designed that is designed to be operated by a single technician in a
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