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A low-cost HPLC method is described for the analysis of aggregation properties of proteins. The method, which utilizes gradient elution, is applicable to a wide range of proteins. The technique enables the determination of the degree and composition of protein aggregation under conditions of protein loading and solution conditions that mimic the effects of the secondary and tertiary structures, such as temperature, pH, ionic strength, and the presence of stabilizers. The protein aggregation assay was successfully employed to demonstrate the denaturation of bovine serum albumin (BSA) by guanidine hydrochloride. Furthermore, the aggregation behavior of BSA and lysozyme in response to the heat treatment of various pH values was found to be different, even though both proteins have the same secondary and tertiary structures.For the first time ever, a player has been fined for landing after being ejected from a helicopter at the U.S. Open: Jhonattan Vegas of Colombia.
Vegas flew into the air after making a birdie putt on the Par 4, 12th hole at the U.S. Open.
Vegas started his descent to the ground from an altitude of 12,000 feet. So did fellow Colombian player Sergio Santos, whose mistake of omission could have ended in Vegas’s walking away from the tournament with his 3-under lead intact.
Vegas took the rough right of the green on the 18th hole. He lined up on the fairway before stepping up to the ball. The ball got slightly offset right of the ball mark, which pulled it slightly to the left. The ball moved so much that it landed behind the ball mark, in the rough, which caused him to jump and then go tumbling through the air.
After the ball landed in the rough, a U.S. Open official and a ball boy immediately raced to the spot where the bounce was, and the ball boy started pulling in the rough, most of which was on the left side of the green.
The balls that were there were all from other players, so the rules official, who was walking on the opposite side of the
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UNITED STATES of America, Plaintiff-Appellee,v.Frank Anthony FLOREZ, Defendant-Appellant.
No. 94-50251.
United States Court of Appeals, Ninth Circuit.
Argued and Submitted June 6, 1995.Decided July 19, 1995.
Before: CANBY, LEAVY and T.G. NELSON, Circuit Judges.
1
MEMORANDUM*
2
Frank Florez appeals his conviction for possession with intent to distribute marijuana in violation of 21 U.S.C. Sec. 841(a)(1). He argues that the district court erred by granting the government’s motion in limine and excluding his expert witness. We have jurisdiction under 28 U.S.C. Sec. 1291, and we affirm.
3
We review a district court’s admission of expert testimony for an abuse of discretion. United States v. Lowe, 860 F.2d 1370, 1376 (9th Cir.1988). We conclude that the district court did not abuse its discretion by excluding Florez’s expert witness. The district court rejected the testimony of the expert on the grounds that his conclusions were not supported by scientific evidence, that the conclusions were not based on sufficient facts or data, and that the expert lacked sufficient background to be qualified as an expert. The district court has “broad discretion to determine the admissibility of expert testimony.” Id. at
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